This MSW thesis explores workplace value differences as a predictor of healthcare worker professional quality of life. Secondary data analysis was performed on data previously collected on hospice and palliative care workers in a 2013 Pan-Canadian Study. The fit of a theoretically informed model to the dataset was analysed, exploring empirical support that healthcare worker value differences within the workplace accounts for a significant part of the variance in their levels professional quality of life. In the Pan-Canadian study, measures of professional quality of life included the Professional Quality of Life Scale (ProQoL). Additionally, the Nursing Unit Cultural Assessment Tool (NUCAT) was also used. This study reviews the pertinent literature, articulates the model suggested above by exploring existing evidence and theory, and tested the model fit using a structural equation model. Understanding the impact that workplace factors have on healthcare worker professional quality of life is an important area of research. Further research utilizing structural equation modeling may help increase our understanding of the multi-factorial nature of professional quality of life measures and its impact on workers, patients, and families.