The present study evaluated the awareness and knowledge of the Canadian 24-Hour Movement Guidelines in rural and remote communities, and explored how to adapt guideline communication material to improve their impact and relevance. People living in rural and remote communities completed a cross-sectional survey (N = 76) and individual interviews (N = 12) to explore what is known about the guidelines and provide recommendations to help adapt the materials to local context. Overall, most participants were aware of the guidelines (44.7%) and had moderate knowledge of recommendations and dosages (64.5%). Deductive thematic analysis was used to code the barriers and facilitators to meeting guideline recommendations and map them onto the Behaviour Change Wheel. Recommendations for adapting the guidelines were created which included: providing additional information on being active outdoors and at home, information discerning sedentary behaviour and inactivity, and tips to combat seasonal changes in daylight.