Moringa oleifera is a tropical tree with nutritious, anti-inflammatory, and antimicrobial properties. Moringa seeds have been studied for their ability to purify water, however roots have not. This study identified the nutrient composition of Moringa roots grown in a greenhouse, and tested whether the roots improved water quality. Moringa roots were dried, powdered and added to contaminated water to test their impact on E. coli, pH, turbidity, and electrical conductivity. The chemical composition of Moringa roots were measured using ICP-MS. The five main elements observed were potassium, phosphorus, magnesium, sodium and calcium. None of the elements extracted were of health concern for drinking water quality. Electrical conductivity and pH remained within drinking water quality guidelines. Moringa root powder resulted in a significant increase in turbidity. Moringa concentration of 600 mg/L removed up to 87% of E. coli in water. Moringa root powder shows some potential as a point-of-use water treatment.