Ascaphus truei, an ancient species of frog, migrated into British Columbia, Canada, following the last ice age. A. truei is of conservation interest because forestry practices, and the associated infrastructure, may reduce habitat quality. There is also concern that a warmer and drier climate will change the distribution and abundance of this species. I used two genetic methods, microsatellites and next-generation sequencing, to compare the genetic diversity of A. truei from the northern extent of its range in British Columbia, CA to southern Washington, USA. Both methods suggested a dramatic reduction in diversity across the northern portion of the species’ range. The phylogeography suggests a northern range expansion from a single refugium. I used DNA metabarcoding to compare the gut contents of larvae across three stream reaches and two development classes near the northern extent of A. truei's range. Gut contents differed between stream reach but did not differ among development class. Wetted width and ultimately stream volume may influence differences in gut content among stream reaches. I also quantified the relationship between an index of larvae density and environmental factors hypothesized to influence population density near the northern extent of the range. I assessed the segregation of larvae at various developmental stages. Wetted width and wetted depth correlated with differences in the abundance of larvae. Older developmental stages were captured in stream reaches with greater slopes than younger stages. Management should minimize modifications of stream structure, such as in-stream siltation due to road building. We should maintain habitat connectivity and gene flow to ensure the continued migration and adaptive capacity of A. truei.
