Glass rod: A glass rod is required to spread the mounting solution on the slide. Wheaton Balsam bottle: This bottle is used to hold the mounting solution and has a special top which. helps prevent contamination of the solution. A glass rod is included for dispensing the solution. Optical Equipment A light field microscope fitted with phase contrast accessories and capable of achieving a magnification of 400X-450X is required. The following parts and accessories must be available with this unit: 10X-Huygenian eye-piece - Koehler illumination - mechanical stage - Abbe or Zernike condensor fitted with a phase ring with a numerical aperture (N.A.) equal to or greater than the N.A. of the objective - 40-45X positive phase contrast achromatic objective (NVA. 0.65 - 0.75) - phase ring centering telescope - Porton reticle (graticule) - stage micrometer (2 mm divided into units of 0.01 mm) (iii) Details of Cassiar Microscope - manufacturer and model: Vickers Instruments Ltd. (U.K.), model M15C microscope - projection: inclined binocular head with high efficiency prism, complan wide field compensating eye-pieces - type of illumination: phase contrast, 98% absorption with -40X objective positive phase. Standard research grade phase contrast adjustable condenser unit - manufacturer's objective type #: Vickers 21485 + Ph.2 N.A. 0.70 working distance 0.68 mm